A NEW VALIDATED RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF CEPHALEXIN AND PROBENECIDIN IN BULK AND COMBINED TABLET DOSAGE FORMS.

kumaraswamy gandla

Abstract


The proposed work accurate and precise RP-HPLC method was developed for the simultaneous estimation of  of Probenecid and Cephalexin, in tablet dosage form. The separation was achieved on a Kromasil C18 (4.6 x 150mm, 5µm) column using a mixture of Acetonitrile: Phosphate Buffer pH 3.2 (70:30v/v) as the mobile phase at a flow rate of 1.0 mL/min, the detection was carried out at 237 nm. The retention time of the Probenecid and Cephalexin was 2.017, 2.352 ±0.01 min respectively. The method produce linear responses in the concentration range of 0.5-5.0 µg/ml of Probenecid and 50-250 µg/ml of Cephalexin. The method precision for the determination of assay was below 2.0%RSD. The method is useful in the quality control of bulk and pharmaceutical formulations.


Keywords


Probenecid, Cephalexin, RP-HPLC, validation.

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References


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DOI: http://dx.doi.org/10.24018/10.24018/iijmps.2018.v1i1.22

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