A NEW VALIDATED RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF AZILSARTAN MEDOXIMIL AND AMLODIPINE IN BULK AND COMBINED TABLET DOSAGE FORMS

kumaraswamy gandla

Abstract


A accurate and precise RP-HPLC has been developed for the validation of Azilsartan medoxilmil and Amlodipine in bulk and combined tablet dosage forms. Separation was achieved on a Primesil (4.6 × 250mm, 5µm particle size) 5µ column using a mixture of Methanol: Acetonitrile: Water 60:20:20v/v as the mobile phase at a flow rate of 1.0 mL/min, the detection was carried out at 230nm. The retention time of the Azilsartan and Amlodipine was 2.813, 3.886 respectively. The method produce linear responses in the concentration range of 10-50µg/ml of Azilsartan and 66.6-330µg/ml of Amlodipine. The method precision for the determination of assay was below 1%RSD. The method is useful in the quality control of bulk and pharmaceutical formulations.


Keywords


Azilsartan , Amlodipine; Tablet dosage forms; RP-HPLC, Method development and validation.

Full Text:

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DOI: http://dx.doi.org/10.24018/10.24018/iijmps.2018.v1i1.22

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