Protective effect of ellagic acid from Punica granatum and vitamin C from Citrus limon juices on malathion induced oxidative stress in rats liver and kidney

Rahul Gupta, Arzoo Pannu, Shikha Goswami, Dhirender Kaushik


Malathion, an organophosphate compound has been proved to play a major role in tissue damage by inducing oxidative stress via generation of free radical. In this study, we examined the consequence of exposure to organophosphate compound malathion of commercial grade and evaluated the protective effect of ellagic acid, vitamin c present in pomegranate and lemon juices respectively. Malathion was administered orally with saline 400mg/kg body weight (BW) daily for 7 days against the respectively juices containing 100mg/kg, 200mg/kg of ellagic acid and vitamin c for 7 and 14 days. At the end of the experiments results showed that under the influence of pesticides, the decrease in the body weight, SOD, CAT, MDA and GSH of rats pesticides treated group compared with normal group and antioxidant treated group, biochemical parameter and histopathology of liver and kidney were estimated. The present study thus gives an insight ill-effect of the malathion and protective role of juices containing antioxidants in minimizing those effects.


Malathion, Oxidative stress, Free radical, Liver, Kidney


Mansour SA, Mossa AH, Heikal TM. Cytogenetic and hormonal adulteration in rats exposed to recommended “safe doses” of spinosad and malathion Insecticides. Int J Agric Biol. 2008; 10: 9-14.

Tuzmen N, Candan N, Kaya E, Demiryas N. Biochemical effect of chlorphyriphos and deltamethrin on altered antioxidative defense mechanism and lipid peroxidation in rat liver. Cell Biochem Funct. 2008; 26:119-124.

Tuzmen N, Candan N, Kaya E. The evaluation of altered antioxidative defense mechanism and anticholinestrase activity in rat brain exposed to chlorphyrifos, deltamethrin, and their combination. Toxicology Mechanisms Met. 2007; 17: 535-540.

Khan PK and Sinha SP. Vitamin C mediated amelioration of pesticide genotoxicity in murine spermatocytes, Cytobios 1994; 80(323): 199-204.

Ogutcu A, Suludere Z, Kalender Y. Dichlorvos-induced hepatotoxicity in rats and the protective effects of vitamin C and E. Environ Toxicol Pharmacol. 2008; 26: 355-361.

Hernandez AF, Parron T, Tsatsakis AM, Requena M, Alarcon R and Guarnido OL. Toxic effects of mixtures at a molecular level: Their relevance to human health. Toxicol. 2012; 51010: 1-10.

Agnihotri MS, Pande SK, Verma H. Immunological significance of negative Mantoux test in tuberculous patients. Ind J Tub. 1977; 24 (2): 62.

Agarwal HC, Pillai MK, Yadav DV, Menon KB and Gupta RK. Residues of DDT and its metabolites in human blood samples in Delhi, India. Bull. World Health Org. 1976; 54: 349-351.

Slorach SA and Vaz R. Assessment of Human Exposure to Selected Organochlorine Compounds through Biological Monitoring, Swedish National Food Administration, Uppsala, Sweden. 1983.

Abo-Zeid MM, El-Barouty G, Abdel-Reheim E, Malathion disposition in dermally and orally treated rats and its impact on the blood serum acetylcholine esterase and protein profile. J Env Sci Health.1993; B28 (4): 413-430.

Olajos EJ, Olson CT and Salem H. Evaluation of neutralized chemical agent identification sets (CAIS) for skin injury with an overview of the vesicant potential of agent degradation products. J Appl Toxicol. 1998; 18(6): 409-20.

Kumar SV, Fareedullah M, Sudhakar Y, Venkteswarlu B and Kumar EA. Current review on organophosphorus poisoning. Arch Appl Sci Res. 2010; 2(4): 199-215.

U.S. Environmental Protection agency. Pesticide Market Estimate: 2006-2007 office of pesticide programs, Washington DC. 2011.

Possamai FP, Fortunato JJ, and Feier G, Agostinho FR, Quevedo J, Filho DW, Dal-Pizzol F. Oxidative stress after acute and sub-chronic malathion intoxication in Wistar rats. Environ Toxicol Pharmacol. 2007; 23: 198–204.

Lukaszewicz-hussain A. Role of oxidative stress in organophosphate insecticide-Short review. Pestic Biochem Physiol. 2010; 98:145-150.

John S, Kale M, Rathore N, Bhatnagar D. Protective effect of vitamin E in dimethoate and malathion induced oxidative stress in rat erythrocytes. J Nutr Biochem. 2001; 12: 500–504.

Banerjee BD, Seth V and Bhattacharya A. Biochemical effects of some pesticides on lipid peroxidation and free-radical scavengers. Toxicol Let. 1999; 107: 33-47.

Al-Othman AM, Al-Numair KS, El-Desoky GE, Yusuf K, Al-Othmann ZA, Aboul-Soud MAM and Giesy JP. Protection of α-tocopherol and selenium against acute effects of malathion on liver and kidney of rats. Afr J Pharm Pharmacol. 2011; 5(10):1263-1271.

Rezg R, Mornagui B, El-Fazaa S, Gharbi N. Caffeic acid attenuates malathion induced metabolic disruption in rat liver, involvement of acetylcholinesterase activity. Toxicology. 2008; 250: 27–31.

Kumar SV, Fareedullah M, Sudhakar Y, Venkteswarlu B, Kumar EA. Current review on organophosphorus poisoning. Arch Appl Sci Res. 2010; 2(4): 199-215.

Hazarika A, Sarkar SN and Hajare S. Influence of malathion pretreatment on the toxicity of anilofos in male rats: a biochemical interaction study. Toxicology. 2003; 185: 1-8.

Cemek M, Buyukben A, Buyukokuroglu ME, Aymelek F, Tur L. Protective roles of vitamin E (a-tocopherol), selenium and vitamin E plus selenium in organophosphate toxicity in vivo: A comparative study. Pestic Biochem Physiol. 2010:96; 113–118.

Kwong TC. Organophosphate pesticides: biochemistry and clinical toxicology, Ther. Drug Monit. 2002; 24: 144–149.

Sevgiler Y, Piner P, Durmaz H, Uner N. Effects of N-acetylcysteine on oxidative responses in the liver of fenthion exposed Cyprinus carpio, Pestic Biochem Physiol. 2007:87; 248–254.

Khan SM. Protective effect of black tea extract on the levels of lipid peroxidation and antioxidant enzymes in liver of mice with pesticide-induced liver injury. Cell Biochem and funct. 2006; 24:327-332

Khan SM and Kour G. Subacute oral toxicity of chlorpyriphos and protective effect of green tea extract. Pesti Bio Physio. 2007; 89:118 -123.

Guo C, Wei J, Yang J, Xu J, Pang W, Jiang Y. Pomegranate juice is potentially better than apple juice in improving antioxidant function in elderly subjects. Nutr Res. 2008; 28: 72-77.

Ahmed RS, Seth V, Pasha ST, Banerjee BD. Influence of dietary ginger (Zingiber Officinales Rose) on oxidative stress induced by malathion in rats. Food Chem Toxicol. 2000; 38:443-450.

Kalender S, Uzun FG, Durak D, Demir F, Kalender Y. Malathion-induced hepatotoxicity in rats: The effects of vitamins C and E. Food Chem Toxicol. 2010; 48: 633–638.

Yadav P, Jadhav S, Kumar V, Kaul KK, Pant SC, Flora SJS. Protective efficacy of 2-PAMCl, atropine and curcumin against dichlorvos induced toxicity in rats. Interdisciplinary Toxicol. 2012; 5: 1-5.

Banerjee BD, Seth V, Bhattacharya A, Pasha ST, Chakraborty AK. Biochemical effects of some pesticides on the lipid peroxidation and free-radical scavengers. Toxicol Lett. 1999; 107: 33-47.

Banerjee BD, Pasha ST, Hussain QZ, Koner BC, Ray A. A comparative evaluation of immunotoxicity of malathion after subchronic exposure in experimental animals. Indian J Exp Biol. 1998: 36: 273-282.

Ranjbar A, Pasalar P, Abdollahi M. Induction of oxidative stress and AChE inhibition in organophosphorous pesticide manufacturing workers. Hum. Exp. Toxicol. 2002; 21: 179–182.

Kovasic P. Mechanism of organophosphates (nerve gases and pesticides) and antidotes: electron transfer and oxidative stress. Curr Med Chem. 2003; 10: 2705–2709.

Vidyasagar J, Karunakar N, Reddy MS, Rajnarayana K, Surender T, Krishna DR. Oxidative stress and antioxidant status in acute organophosphorous insecticide poisoning. Indian J Pharmacol. 2004; 36: 76-9.

Abdollahi M, Ranjbar A, Shadnia S, Nikfar S, Rezaie OE. Pesticides and oxidative stress: A review .Med Sci Monit. 2004; 10:141-147.

Uzun FG, Kalender S, Durak D. Malathion-induced testicular toxicity in male rats and the protective effect of vitamins C and E. Food Chem Toxicol. 2009; 47: 1903–1908.

Gupta PK. Malathion induced biochemical changes in rat. Acta Pharmacol. Toxicol. (Copenh). 1974; 35: 191–194

Jain N, Sharma P, Sharma N and Joshi SC. Haemato-Biochemical Profile following subacute toxicity of malathion in male albino rat. Pharmacol online. 2009; 2: 500-506.

Uzunhisarcikli M, Kalender Y, Dirican K, Kalender S, Ogutcu A, Buyukkomurcu F. Acute, subacute and subchronic administration of methyl parathion-induced testicular damage in male rats and protective role of vitamin C and E. Pestic Biochem Physiol. 2007; 87:115-122.

Uzunhisarcikli M, Kalender Y. Protective effects of vitamins C and E against hepatotoxicity induced by methyl parathion in rats. Ecotox Environ Safe. 2011; 74: 2112–2118.



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