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The proposed work accurate and precise RP-HPLC method was developed for the simultaneous estimation of Â of Probenecid and Cephalexin, in tablet dosage form. The separation was achieved on a Kromasil C18 (4.6 x 150mm, 5Âµm) column using a mixture of Acetonitrile: Phosphate Buffer pH 3.2 (70:30v/v) as the mobile phase at a flow rate of 1.0 mL/min, the detection was carried out at 237 nm. The retention time of the Probenecid and Cephalexin was 2.017, 2.352 Â±0.01 min respectively. The method produce linear responses in the concentration range of 0.5-5.0 Âµg/ml of Probenecid and 50-250 Âµg/ml of Cephalexin. The method precision for the determination of assay was below 2.0%RSD. The method is useful in the quality control of bulk and pharmaceutical formulations.
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