Main Article Content
A accurate and precise RP-HPLC has been developed for the validation of Azilsartan medoxilmil and Amlodipine in bulk and combined tablet dosage forms. Separation was achieved on a Primesil (4.6 Ã— 250mm, 5Âµm particle size) 5Âµ column using a mixture of Methanol: Acetonitrile: Water 60:20:20v/v as the mobile phase at a flow rate of 1.0 mL/min, the detection was carried out at 230nm. The retention time of the Azilsartan and Amlodipine was 2.813, 3.886 respectively. The method produce linear responses in the concentration range of 10-50Âµg/ml of Azilsartan and 66.6-330Âµg/ml of Amlodipine. The method precision for the determination of assay was below 1%RSD. The method is useful in the quality control of bulk and pharmaceutical formulations.
Dr. Kealey and P.J Haines, Analytical Chemistry, 1stedition, Bios Publisher, (2002), PP 1-7.
A.BraithWait and F.J.Smith, Chromatographic Methods, 5thedition, Kluwer Academic Publisher, (1996), PP 1-2.
Andrea Weston and Phyllisr. Brown, HPLC Principle and Practice, 1st edition,
Academic press, (1997), PP 24-37.
Yuri Kazakevich and Rosario Lobrutto, HPLC for Pharmaceutical Scientists, 1stedition, Wiley Interscience A JohnWiley & Sons, Inc., Publication, (2007), PP 15-23.
Chromatography, (online). URL:http://en.wikipedia.org/wiki/Chromatography.
Meyer V.R. Practical High-Performance Liquid Chromatography, 4th Ed. England, John Wiley & Sons Ltd, (2004), PP 7-8.
Sahajwalla CG a new drug development, vol 141, Marcel Dekker Inc., New York, (2004), PP 421â€“426.
Introduction to Column. (Online),URL:http://amitpatel745.topcities.com/index_files/study/column care.pdf
Detectors used in HPLC (online )URL:http://wiki.answers.com/Q/What_detectors_are_used_in_HPLC
Detectors (online) ,URL:http://hplc.chem.shu.edu/NEW/HPLC_Book/Detectors/det_uvda.html
Detectors (online) ,URL:http://www.dionex.com/enus/webdocs/64842-31644-02_PDA-100.pdf