ANTI-INFLAMMATORY AND BACTERIAL ACTIVITY OF VARIOUS EXTRACTS OF MOMORDICA CHARANTIA

Main Article Content

RENUKA GUDEPU
GUNASWETHA KURAGANTI
SWETHA VIDDAMURI

Abstract

Objective: The present investigation was carried out to evaluate the antibacterial activity of different plant parts (leaf, fruit, seed, and stem) of Momordica charantia.


Materials and Methods: The plant parts were subjected to successive extraction using six different solvents viz., Distilled water, Ethanol, Methanol, Acetone, Hexane & Petroleum ether. The antibacterial activity was determined by Agar–well diffusion method against 6 Gram-positive bacterial strains viz., Staphylococcus aureus, Staphylococcus epidermidis, Bacillus subtilis, Bacillus cereus, Enterococcus feacalis & Micrococcus luteus and 7 Gram-negative bacterial strains viz., Escherichia coli, Klebsiella pneumoniae, Proteus vulgaris, Proteus mirabilis, Enterobacter aerogenes, Alcaligenes faecalis & Pseudomonas aeuriginosa. Among the various extracts, aqueous followed by ethanol extracts were found to be more effective against all the bacteria.


Results: The plant extracts were more active against Gram-positive bacteria than Gram-negative. The most resistant bacteria were Alcaligenes faecalis & Pseudomonas aeuriginosa while Staphylococcus aureus, together with Escherichia coli appeared to be the most susceptible. The aqueous leaf extract exhibited remarkable antibacterial activity against S.aureus (26 mm)which was comparable with standard Ciprofloxacin. The results obtained appeared to confirm the antibacterial potential of various extracts of M. charantia parts investigated, and their usefulness in the treatment of bacterial diseases. Further to study the anti-proliferative properties of M. charantia MTT assay was conducted on RAW 264.7 cells using LPS as a positive control. The result obtained shows a 100ug/ml concentration of extract to have an equivalent anti-proliferative effect as lipopolysaccharide.


Conclusion: Previous studies have demonstrated a close relationship between inflammation and several diseases. This study conducted demonstrates the therapeutic

Downloads

Download data is not yet available.

Article Details

How to Cite
RENUKA GUDEPU, GUNASWETHA KURAGANTI, & SWETHA VIDDAMURI. (2022). ANTI-INFLAMMATORY AND BACTERIAL ACTIVITY OF VARIOUS EXTRACTS OF MOMORDICA CHARANTIA . Innovat International Journal Of Medical & Pharmaceutical Sciences, 7(4), 14–19. Retrieved from https://innovatpublisher.com/index.php/iijmps/article/view/184
Section
Original Article(s)

References

Farzianpour F, Bakhtiari A, Mohammadi M, Khosravizadeh O, Mossavi H, Mohseni M et al. Analysis of nosocomial infections in selected teaching hospitals, Qazvin, Iran. Health. 2014;06(18):2425-32. doi: 10.4236/health.2014.618279.

Islam QR, Shahidullah M, Islam MZ, Mridha MAA, Akter S. Bacterial profile of neonatal septicemia and antibiotic susceptibility pattern of the isolates in tertiary care hospital, Dhaka, Bangladesh. Bangladesh J Child Health. 2019;43(1):35-40. doi: 10.3329/bjch.v43i1.41216.

Zielnik-Jurkiewicz B, Bielicka A. Antibiotic resistance of Streptococcus pneumoniae in children with acute otitis media treatment failure. Int J Pediatr Otorhinolaryngol. 2015;79(12):2129-33. doi: 10.1016/j.ijporl.2015.09.030, PMID 26454530.

Bandow JE, Brötz H, Leichert LI, Labischinski H, Hecker M. Proteomic approach to understanding antibiotic action. Antimicrob Agents Chemother. 2003;47(3):948-55. doi: 10.1128/AAC.47.3.948-955.2003, PMID 12604526.

Zare G, Diker NY, Arituluk ZC, Cankaya II. Chelidonium majus L. (Papaveraceae) morphology, anatomy and traditional medicinal uses in Turkey. J Pharm Istanb Univ. 2021;51(1):123-32. doi: 10.26650/IstanbulJPharm.2020.0074.

Scazzocchio F, Cometa MF, Tomassini L, Palmery M. Antibacterial activity of Hydrastis canadensis extract and its major isolated alkaloids. Planta Med. 2001;67(6):561-4. doi: 10.1055/s-2001-16493, PMID 11509983.

Mohammadi A, Nazari H, Imani S, Amrollahi H. Antifungal activities and chemical composition of some medicinal plants. J Mycol Med. 2014;24(2):e1-8. doi: 10.1016/j.mycmed.2014.02.006, PMID 24768063.8. Soo May L, Sanip Z, Ahmed Shokri A, Abdul Kadir A, Md Lazin MR. The effects of Momordica charantia (bitter melon) supplementation in patients with primary knee osteoarthritis: A single-blinded, randomized controlled trial. Complement Ther Clin Pract. 2018;32:181-6. doi: 10.1016/j.ctcp.2018.06.012, PMID 30057048.

Mathabe MC, Nikolova RV, Lall N, Nyazema NZ. Antibacterial activities of medicinal plants used for the treatment of diarrhoea in Limpopo Province, South Africa. J Ethnopharmacol. 2006;105(1-2):286-93. doi: 10.1016/j.jep.2006.01.029, PMID 16545928.

Cos P, Vlietinck AJ, Berghe DV, Maes L. Anti-infective potential of natural products: how to develop a stronger in vitro ’proof-of-concept’. J Ethnopharmacol. 2006 July 19;106(3):290-302. doi: 10.1016/j.jep.2006.04.003, PMID 16698208.

Duraipandiyan V, Ayyanar M, Ignacimuthu S. Antimicrobial activity of some ethnomedicinal plants used by Paliyar tribe from Tamil Nadu, India. BMC Complement Altern Med. 2006;6(1):35. doi: 10.1186/1472-6882-6-35, PMID 17042964.

Parekh J, Chanda S. In vitro antimicrobial activity and phytochemical analysis of some Indian medicinal plants. Turk J Biol. 2007;31(1):53-8.

Grover JK, Yadav SP. Pharmacological actions and potential uses of Momordica charantia: a review. J Ethnopharmacol. 2004;93(1):123-32. doi: 10.1016/j.jep.2004.03.035, PMID 15182917.

Ahamad J, Amin S, R. Mir SR. Momordica charantia Linn.(Cucurbitaceae): review on phytochemistry and pharmacology. Phytochemistry. 2017;11(2):53-65. doi: 10.3923/rjphyto.2017.53.65.

Adeshina GO, Olayinka BO, Mohammed A, Ibrahim YK, Onaolapo JA. Antistaphylococcal activity of N-butanol and aqueous sub-fractions of Alchornea cordifolia leaves. Br J Pharm Res. 2012;2(4):238-48. doi: 10.9734/BJPR/2014/1727.

Vimala G, Gricilda Shoba F. A review on antiulcer activity of few Indian medicinal plants. Int J Microbiol. 2014 May 25;2014:519590. doi: 10.1155/2014/519590, PMID 24971094.

Tabassum N, Hamdani M. Plants used to treat skin diseases. Pharmacogn Rev. 2014;8(15):52-60. doi: 10.4103/0973-7847.125531, PMID 24600196.

Purushothaman YA, Gunaseelan SI, Vijayakumar SD. Spilanthes Acmella and its medicinal uses-a review. Asian J Pharm Clin Res. 2018;11(6):45-9. doi: 10.22159/ajpcr.2018.v11i6.24697.

Saeed SA, Tariq PE. Antibacterial activities of Mentha piperita, Pisum sativum and Momordica charantia. Pak J Bot. 2005;37(4):997.

Bibitha B, Jisha VK, Salitha CV, Mohan S, Valsa AK. Antibacterial activity of different plant extracts. Short communication. Indian J Microbiol. 2002;42(6). doi: 10.12691/ajfst-2-1-2.

Van Vuuren SF. Antimicrobial activity of South African medicinal plants. J Ethnopharmacol. 2008;119(3):462-72. doi: 10.1016/j.jep.2008.05.038, PMID 18582553.

Parekh J, Chanda S. In-vitro antimicrobial activities of extracts of Launaea procumbens roxb.(Labiateae), Vitis vinifera l.(Vitaceae) and Cyperus rotundus l. Afr J Biomed Res. 2006;9(2). doi: 10.4314/ajbr.v9i2.48780.

Balows A. Manual of clinical microbiology. 8th ed Murray PR, Baron EJ, Jorgenson JH, Pfaller MA, Yolken RH, editors. ASM Press, 2003. 2113 p, 2 vol+ subject & author indices, ISBN: 1-555810255-4, US $189.95. Diagn. Microbiol. Infect. Dis. 2003 Dec;47(4):625. doi: 10.1016/S0732-8893(03)00160-3.

Aliero AA, Afolayan AJ. Antimicrobial activity of Solanum tomentosum. Afr J Biotechnol. 2006;5(4):369-72.

Alam MA, Uddin R, Subhan N, Rahman MM, Jain P, Reza HM. Beneficial role of bitter melon supplementation in obesity and related complications in metabolic syndrome. J Lipids. 2015;2015:496169. doi: 10.1155/2015/496169, PMID 25650336