Main Article Content



Objective: To find out the effect of biofertilizers on the growth and yield of Tribulus terrestris by using vermicompost, vesicular mycorrhiza (VAM), phosphate solubilizing bacteria (PBS), nitrogen-fixing bacteria individually and in combination under garden house environment.

Materials and Methods: The plant seedlings were gathered at different intervals on the 30th, 60th, and 90th days later the transplantations. By the inoculation of vermicompost, VAM fungi, and phosphate solubilizing, nitrogen-fixing microorganisms shows in improvement of growth parameters as like plant shoot height, root length, a number of leaves, leaf area, fresh shoot biomass and fresh root biomass, dry shoot biomass and dry root biomass, chlorophyll content, reducing sugar and non-reducing sugar, starch, protein and lipid content in the root samples.

Results: The result highlights the significance of biofertilizer inoculations for the fast development of seedlings of a medicinal plant (Tribulus terrestris) grown in a garden house and shows the benefit of inoculating soil with fewer microbial inhabitants with aboriginal microbes.

Conclusion: Overall results revealed that the biofertilizers are the better supplements that can improve and show the maximum growth and yield with all inoculations than the individual.


Download data is not yet available.

Article Details

How to Cite
SHAKILA PARVIN J, VENKATESWARULU N, & VIJAYA T. (2021). A STUDY ON EFFECT OF VERMICOMPOST VAM FUNGI NITROGEN FIXING BACTERIA AND PHOSPHATE SOLUBILIZING BACTERIA ON GROWTH PARAMETERS AND PHYSIOLOGY OF TRIBULUS TERRESTRIS L. Innovat International Journal Of Medical & Pharmaceutical Sciences, 7(3), 17–27. Retrieved from https://innovatpublisher.com/index.php/iijmps/article/view/186
Original Article(s)


Akhzaria D, Kalantaria N, Mahdavib SH. Studying the effects of mycorrhiza and vermicompost fertilizers on the growth and physiological traits of vetiver Grass (Chrysopogon zizanioides L.). Desert. 2018;23(1):57-62.

Hibah Shahin Fathimaa H, Nalina L, Rajamani K, Balachander D, Thiribhuvanamala. Effect of different media and bioinoculants on growth and yield characters of vetiver (Chrysopogon zizanioides (L.) Roberty). JPP. 2019;8(4):145-8.

Shaimaa A, Mohamed Massoud ON. Impact of Inoculation with Mycorrhiza and Azotobacter under Different N and P Rates on Growth, Nutrient status, Yield and Some Soil Characteristics of Washington Navel Orange Tree. Middle East J Agric Res. 2017:617-38.

Padmadevi SN, Bai RSM, William SPMP, Sunithakumari K. Organic cultivation of medicinal plants: influence of composted coir pith on the growth and yield of Coleus forskohlii (willd.) Briq. Compost Sci Util. 2016;24(4):266-72. doi: 10.1080/1065657X.2015.1013583.

Wu SC, Cao ZH, Li ZG, Cheung KC, Wong MH. Effects of biofertilizer containing N-fixer, P and K solubilizers and AM fungi on maize growth: A greenhouse trial. Geoderma. 2005;125(1-2):155-66. doi: 10.1016/j.geoderma.2004.07.003.

Bashan Y, Bashan LE. Plant growth-promoting. Encyclopedia. Soils Environ. 2005;1:103-15.

Tarnabi ZM, Iranbakhsh A, Mehregan I, Ahmadvand R. Impact of arbuscular mycorrhizal fungi (AMF) on gene expression of some cell wall and membrane elements of wheat (Triticum aestivum L.) under water deficit using transcriptome analysis. Physiol Mol Biol Plants. 2019:1-11.

Zhu XC, Song FB, Xu HW. Arbuscular mycorrhizae improve low temperature stress in maize via alterations in host water status and photosynthesis. Plant Soil. 2010;331(1-2):129-37. doi: 10.1007/s11104-009-0239-z.

Ahanger MA, Tyagi SR, Wani MR, Ahmad P. Drought tolerance: role of organic osmolytes, growth regulators, and mineral nutrients. In: Ahmad P, Wani MR, editors, Physiological mechanisms and adaptation strategies in plants under changing environment. Vol. 1. New York: Springer; 2014. p. 25-55.

Begum N, Qin C, Ahanger MA, Raza S, Khan MI, Ashraf M et al. Role of arbuscular mycorrhizal fungi in plant growth regulation: implications in abiotic stress tolerance. Front Plant Sci. 2019;10:1068. doi: 10.3389/fpls.2019.01068, PMID 31608075.

Tang Y, Wen G, Li P, Dai C, Han J. Effects of biogas slurry application on crop production and soil properties in a rice–wheat rotation on coastal reclaimed farmland. Water Air Soil Pollut. 2019;230(3):51. doi: 10.1007/s11270-019-4102-4.

Rana A, Joshi M, Prasanna R, Shivay YS, Nain L. Biofortification of wheat through inoculation of plant growth promoting rhizobacteria and cyanobacteria. Eur J Soil Biol. 2012;50:118-26. doi: 10.1016/j.ejsobi.2012.01.005.

Rosas SB, Avanzini G, Carlier E, Pasluosta C, Pastor N, Rovera M. Root colonization and growth promotion of wheat and maize by Pseudomonas aurantiaca SR1. Soil Biol Biochem. 2009;41(9):1802-6. doi: 10.1016/j.soilbio.2008.10.009.

Hungria M, Campo RJ, Souza EM, Pedrosa FO. Inoculation with selected strains of Azospirillum brasilense and A. lipoferum improves yields of maize and wheat in Brazil. Plant Soil. 2010;331(1-2):413-25. doi: 10.1007/s11104-009-0262-0.

Orhan E, Esitken A, Ercisli S, Turan M, Fikrettin S. Effects of plant growth promoting rhizobacteria (PGPR) on yield, growth and nutrient contents in organically growing raspberry. Sci Hortic. 2006;111:38-43.

Çakmakçi R, Dönmez F, Aydın A, Şahin F. Growth promotion of plants by plant growth promoting rhizobacteria under greenhouse and two different field soil conditions. Soil Biol Biochem. 2006;38(6):1482-7. doi: 10.1016/j.soilbio.2005.09.019.

da Silva SF, Olivares FL, Canellas LP. The biostimulant manufactured using diazotrophic endophytic bacteria and humates is effective to increase sugarcane yield. Chem Biol Technol Agric. 2017;4(1):24. doi: 10.1186/s40538-017-0106-8.

Shahid M, Hameed S, Imran A, Ali S, van Elsas JD. Root colonization and growth promotion of sunflower (Helianthusannuus L.) by phosphate solubilizing Enterobacter sp. Fs-11. World J Microbiol Biotechnol. 2012;28(8):2749-58. doi: 10.1007/s11274-012-1086-2, PMID 22806201.

Canellas LP, Balmori DM, Médici LO, Aguiar NO, Campostrini E, Rosa RCC et al. A combination of humic substances and Herbaspirillum seropedicae inoculation enhances the growth of maize (Zea mays L.). Plant Soil. 2013;366(1-2):119-32. doi: 10.1007/s11104-012-1382-5.

Thonar C, Lekfeldt JDS, Cozzolino V, Kundel D, Kulhánek M, Mosimann C et al. Potential of three microbial bio-effectors topromote maize growth and nutrient acquisition from alternative phosphorous fertilizers in contrasting soils. Chem Biol Technol Agric. 2017;4(1):7. doi: 10.1186/s40538-017-0088-6.

Kurrey DK, Sharma R, Lahre MK, Kurrey RL. Effect of Azotobacter on physio-chemical characteristics of soil in onion field. J Pharm Innov J. 2018;7(2):108-13.

Gothandapani S, Sekar S, C Padaria JC. Azotobacter chroococcum: utilization and potential use for agricultural crop production: an overview. Int J Adv Res Biol Sci. 2017;4(3):35-42. doi: 10.22192/ijarbs.2017.04.03.004.