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Background: Diabetes mellitus progression is triggered by oxidative stress, inflammation and pancreatic apoptosis. Therefore, this study investigated effects of Rida herbal bitters (RHB) on oxidative stress, inflammatory response and apoptosis in pancreas of high-fat diet and streptozotocin-induced diabetic rats.

Materials and Methods: Thirty-two (32) male Wistar rats weighing (180 ±20g) were used and diabetes was induced by repeated intraperitoneal injection of streptozotocin (35 mg/kgbw) after feeding the animals with high-fat diet for six weeks. The rats were randomly assigned into four groups, 8rats each. Group I: control; Group II: diabetic; Group III & IV: diabetic rats received 0.3ml RHB and 200mg/kgbwp.o metformin for 28days. Daily food and water intake were recorded daily, body weight and fasting blood glucose levels weekly throughout the experiment. The animals were sacrificed on the last day; blood and pancreas were collected for biochemical investigations.

Results: Diabetic rats exhibited significant (p<0.05) increases in fasting blood glucose (FBG), serum insulin, pancreatic tumor necrosis factor-alpha (TNF-α), caspase-3, malondialdehyde (MDA), and water intake and reductions in pancreatic interleukin-10 (IL-10), B cell lymphoma-2 (Bcl-2), reduced glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), body weight and food intake. Rida herbal bitters administration attenuated all these changes comparable to metformin in most studied parameters.

Conclusion: In the pancreatic tissue, Rida herbal bitters effectively improved pancreatic cells by reducing hyperglycemia through activating endogenous antioxidants, recovering insulin sensitivity, suppressing inflammation and apoptosis in type II diabetic rat model. RHB could be used as novel therapy for diabetes.


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AJAO FOLASADE OMOBOLANLE, & IYEDUPE MARCUS OLAOYE. (2022). RIDA HERBAL BITTERS ATTENUATES PANCREATIC OXIDATIVE STRESS, INFLAMMATION AND APOPTOSIS IN HIGH-FAT DIET/STREPTOZOTOCIN-INDUCED DIABETIC RATS. Innovat International Journal Of Medical & Pharmaceutical Sciences, 7(4), 8–13. Retrieved from https://innovatpublisher.com/index.php/iijmps/article/view/196
Original Article(s)


Ogurtsova K, da Rocha Fernandes JD, Huang Y, Linnenkamp U, Guariguata L, Cho NH et al. IDF Diabetes Atlas: global estimates for the prevalence of diabetes for 2015 and 2040. Diabetes Res Clin Pract. 2017;128:40-50. doi: 10.1016/j.diabres.2017.03.024, PMID 28437734.

Ramtahal R, Khan C, Maharaj-Khan K, Nallamothu S, Hinds A, Dhanoo A et al. Prevalence of self-reported sleep duration and sleep habits in type 2 diabetes patients in South Trinidad. J Epidemiol Glob Health. 2015;5(4);Suppl 1:S35-43. doi: 10.1016/j.jegh.2015.05.003, PMID 26073574.

Mobasseri M, Shirmohammadi M, Amiri T, Vahed N, Hosseini Fard HH, Ghojazadeh M. Prevalence and incidence of type 1 diabetes in the world: A systematic review and meta-analysis. Health Promot Perspect. 2020;10(2):98-115. doi: 10.34172/hpp.2020.18, PMID 32296622.

Khamchan A, Paseephol T, Hanchang W. Protective effect of wax apple (Syzygium samarangense (Blume) Merr. & L.M. Perry) against streptozotocin-induced pancreatic ß-cell damage in diabetic rats. Biomed Pharmacother. 2018;108:634-45. doi: 10.1016/j.biopha.2018.09.072, PMID 30245463.

Yaribeygi H, Atkin SL, Sahebkar A. A review of the molecular mechanisms of hyperglycemia-induced free radical generation leading to oxidative stress. J Cell Physiol. 2019;234(2):1300-12. doi: 10.1002/jcp.27164, PMID 30146696.

Pickering RJ, Rosado CJ, Sharma A, Buksh S, Tate M, de Haan JB. Recent novel approaches to limit oxidative stress and inflammation in diabetic complications. Clin Transl Immunology. 2018;7(4):e1016. doi: 10.1002/cti2.1016, PMID 29713471.

Rehman K, Akash MSH. Mechanism of generation of oxidative stress and pathophysiology of type 2 diabetes mellitus: how are they interlinked? J Cell Biochem. 2017;118(11):3577-85. doi: 10.1002/jcb.26097, PMID 28460155.

Rashid K, Sil PC. Curcumin enhances recovery of pancreatic islets from cellular stress induced inflammation and apoptosis in diabetic rats. Toxicol Appl Pharmacol. 2015;282(3):297-310. doi: 10.1016/j.taap.2014.12.003, PMID 25541178.

Kooti W, Farokhipour M, Asadzadeh Z, Ashtary-Larky D, Asadi-Samani M. The role of medicinal plants in the treatment of diabetes: a systematic review. Electron Phys. 2016;8(1):1832-42. doi: 10.19082/1832, PMID 26955456.

Obasi DC, Ogugua VN, Obasi JN, Okagu IU. Phytochemical, nutritional and anti-nutritional analyses of Ruzu herbal bitters. IOSR JPBS. 2020;15(1):4-17.11. Furman BL. Streptozotocin-induced diabetic models in mice and rats. Curr Protoc Pharmacol. 2015;70, 5.47.1-5.20. doi: 10.1002/0471141755.ph0547s70, PMID 26331889.

Gupta R, Gupta RS. Protective Role of Pterocarpus marsupium in Diabetes-Induced hyperlipidemic Condition. J Complement Integr Med. 2009;6(1):21. doi: 10.2202/1553-3840.1288.

Prasanna Kumar HR, Gowdappa HB, Hosmani T, Urs T. Exocrine dysfunction correlates with endocrinal impairment of pancreas in type 2 diabetes mellitus. Indian J Endocrinol Metab. 2018;22(1):121-25. doi: 10.4103/ijem.IJEM_139_17, PMID 29535950.

Wang L, Chang JH, Buckley AF, Spurney RF. Knockout of TRPC6 promotes insulin resistance and exacerbates glomerular injury in akita mice. Kidney Int. 2019;95(2):321-32. doi: 10.1016/j.kint.2018.09.026, PMID 30665571.

Njagi JM, Ngugi MP, Kibiti CM, Ngeranwa J, Njue W, Gathumbi P et al. Hypoglycemic effect of Helichrysum odoratissimum in alloxan induced diabetic mice. J Phytopharmacol. 2015;4(1):30-3. doi: 10.31254/phyto.2015.4106.

Guo XX, Wang Y, Wang K, Ji BP, Zhou F. Stability of a type 2 diabetes rat model induced by high-fat diet feeding with low-dose streptozotocin injection. J Zhejiang Univ Sci B. 2018;19(7):559-69. doi: 10.1631/jzus.B1700254, PMID 29971994.

Yakubu MT, Uwazie NJ, Igunnu A. Antidiabetic activity of aqueous extract of Senna alata (Fabaceae) fower in alloxaninduced diabetic male rats. Cameroon J Biol Biochem Sci. 2016;24:7-17.

Rizza RA. Pathogenesis of fasting and postprandial hyperglycemia in type 2 diabetes: implications for therapy. Diabetes. 2010;59(11):2697-707. doi: 10.2337/db10-1032, PMID 20705776.

Pari L, Rajarajeswari N. Efficacy of coumarin on hepatic key enzymes of glucose metabolism in chemical induced type 2 diabetic rats. Chem Biol Interact. 2009;181(3):292-6. doi: 10.1016/j.cbi.2009.07.018, PMID 19647726.

Parveen K, Khan MR, Mujeeb M, Siddiqui WA. Protective effects of Pycnogenol on hyperglycemia-induced oxidative damage in the liver of type 2 diabetic rats. Chem Biol Interact. 2010;186(2):219-27. doi: 10.1016/j.cbi.2010.04.023, PMID 20433812.

Akcilar R, Kocak FE, Simsek H, Akcilar A, Bayat Z, Ece E et al. Antidiabetic and hypolipidemic effects of adropinin streoptozotocin-induced type 2 diabetic rats. Bratisl Lek Listy. 2016;117(2):100-5. doi: 10.4149/bll_2016_020, PMID 26830041.

ShaymaaAbdulmalek AE, DoaaAwad MB. Ameliorative effect of curcumin and zinc oxide nanoparticles on multiple mechanisms in obese rats with induced type 2 diabetes. Scientific Rep. 2021;11:20677.

Newsholme P, Cruzat VF, Keane KN, Carlessi R, de Bittencourt Jr PIH. Molecular mechanisms of ROS production and oxidative stress in diabetes. Biochem J. 2016;473(24):4527-50. doi: 10.1042/BCJ20160503C, PMID 27941030.

Pashapoor A, Mashhadyrafie S, Mortazavi P. Ameliorative effect of Myristica fragrans (nutmeg) extract on oxidative status and histology of pancreas in alloxan induced diabetic rats. Folia Morphol. 2020;79(1):113-19. doi: 10.5603/FM.a2019.0052, PMID 31063201.

Wen W, Lin Y, Ti Z. Antidiabetic, antihyperlipidemic, antioxidant, anti-inflammatory activities of ethanolic seed extract of Annona reticulata L. in streptozotocin induced diabetic rats. Front Endocrinol. 2019;10:716. doi: 10.3389/fendo.2019.00716, PMID 31708869.

Al-Quraishy S, Dkhil MA, Abdel Moneim AE. Anti-hyperglycemic activity of selenium nanoparticles in streptozotocininduced diabetic rats. Int J Nanomedicine. 2015;10:6741-56.

Eguchi K, Nagai R. Islet inflammation in type 2 diabetes and physiology. J Clin Invest. 2017;127(1):14-23. doi: 10.1172/JCI88877, PMID 28045399.

Adam SitiH, Giribabu N, Kassim N, Kumar KE, Brahmayya M, Arya A et al. Protective effect of aqueous seed extract of Vitis vinifera against oxidative stress, inflammation and apoptosis in the pancreas of adult male rats with diabetes mellitus. Biomed Pharmacother. 2016;81:439-52. doi: 10.1016/j.biopha.2016.04.032, PMID 27261624.