RIDA HERBAL BITTERS ATTENUATES PANCREATIC OXIDATIVE STRESS, INFLAMMATION AND APOPTOSIS IN HIGH-FAT DIET/STREPTOZOTOCIN-INDUCED DIABETIC RATS

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Published: Jul 1, 2022
Keywords:
Rida herbal bitters Diabetes mellitus Pancreas Oxidative stress Inflammation

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AJAO FOLASADE OMOBOLANLE
Department of Physiology, Faculty of Basic Medical Science, Ladoke Akintola University of Technology, P.M.B. 4000, Ogbomoso, Oyo State, Nigeria.
IYEDUPE MARCUS OLAOYE
Department of Physiology, Faculty of Basic Medical Science, Ladoke Akintola University of Technology, P.M.B. 4000, Ogbomoso, Oyo State, Nigeria.

Abstract

Background: Diabetes mellitus progression is triggered by oxidative stress, inflammation and pancreatic apoptosis. Therefore, this study investigated effects of Rida herbal bitters (RHB) on oxidative stress, inflammatory response and apoptosis in pancreas of high-fat diet and streptozotocin-induced diabetic rats.


Materials and Methods: Thirty-two (32) male Wistar rats weighing (180 ±20g) were used and diabetes was induced by repeated intraperitoneal injection of streptozotocin (35 mg/kgbw) after feeding the animals with high-fat diet for six weeks. The rats were randomly assigned into four groups, 8rats each. Group I: control; Group II: diabetic; Group III & IV: diabetic rats received 0.3ml RHB and 200mg/kgbwp.o metformin for 28days. Daily food and water intake were recorded daily, body weight and fasting blood glucose levels weekly throughout the experiment. The animals were sacrificed on the last day; blood and pancreas were collected for biochemical investigations.


Results: Diabetic rats exhibited significant (p<0.05) increases in fasting blood glucose (FBG), serum insulin, pancreatic tumor necrosis factor-alpha (TNF-α), caspase-3, malondialdehyde (MDA), and water intake and reductions in pancreatic interleukin-10 (IL-10), B cell lymphoma-2 (Bcl-2), reduced glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), body weight and food intake. Rida herbal bitters administration attenuated all these changes comparable to metformin in most studied parameters.


Conclusion: In the pancreatic tissue, Rida herbal bitters effectively improved pancreatic cells by reducing hyperglycemia through activating endogenous antioxidants, recovering insulin sensitivity, suppressing inflammation and apoptosis in type II diabetic rat model. RHB could be used as novel therapy for diabetes.

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AJAO FOLASADE OMOBOLANLE, & IYEDUPE MARCUS OLAOYE. (2022). RIDA HERBAL BITTERS ATTENUATES PANCREATIC OXIDATIVE STRESS, INFLAMMATION AND APOPTOSIS IN HIGH-FAT DIET/STREPTOZOTOCIN-INDUCED DIABETIC RATS. Innovat International Journal Of Medical & Pharmaceutical Sciences, 7(4), 8–13. Retrieved from https://innovatpublisher.com/index.php/iijmps/article/view/196
Issue
Vol 7 Issue 4 2022
Section
Original Article(s)

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