CHEMICAL INVESTIGATION, ISOLATION AND BIOACTIVITY TESTING OF PHYTOMOLECULES FROM CYPERUS SCARIOSUS
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Objective: The present study was carried out to extract, isolate and evaluate the antimicrobial activity of the root and aerial part of Cyperus scariosus (Nagarmotha).
Material and Methods: In this research, cyperus oil was extracted from the root by various distillation techniques such as Water/hydro distillation (Clevenger apparatus), and Steam distillation (Boiler operated). The root and aerial part of Nagarmotha was extracted in different solvents such as hexane, aqueous, and ethanol and evaluated for antimicrobial activity at different concentrations against 6 gram-positive (Staphylococcus aureus, Streptococcus epidermidis, Bacillus subtilis, Yersinia enterocolitica, Salmonella typhimurium, Enterobacter aerogenes ), 1 gram-negative (E.Coli ) bacterial strains and 3 fungal strains (Candida albicans, Aspergillus niger, Aspergillus flavus ). Amikacin and fluconazole (100 μg/ml) were taken as standard drugs.
Result and Discussion: The % yield of oil was 0.84 % (v/w) by both methods. The Isolated compound was characterized by various spectroscopic techniques such as GC, GC-MS, and 1H NMR and established as (-)-ISOLONGIFOLEN-9-ONE. Root and aerial extract in different solvents showed mild antimicrobial activity at 100 μg/ml. Pure oil (NM Oil) showed mild to moderate antibacterial activity at 100 μg/ml against gram-positive bacteria but it was not shown any activity against gram-negative bacteria. Pure oil was also shown very good activity against fungal strains such as Candida albicans and Aspergillus flavus when compared with the standard drug Fluconazole.
Conclusion: The present study showed that the pure oil obtained from the Cyperus scariosus has the potential to diminish microbial growth and can be effective in the treatment of microbial diseases.
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