NOVEL STABILTY INDICATING RP-HPLC METHOD SIMULTANEOUS DETERMINATION OF SOFOSBUVIR AND VELPATASVIR IN BULK AND COMBINED TABLET DOSAGE FORMS

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Gandla Kumaraswamy
K Pranay
M Rajkumar
R Lalitha

Abstract

A accurate and precise RP-HPLC method has been developed for the validated of Sofosbuvir and Velpatasvir in bulk and combined Tablet dosage form. Separation was carried out on a Primesil C18 (4.6 x 250mm, 5µm) column using a mixture of Acetonitrile: 0.1%perchloricacid  (50:50 v/v) as the mobile phase at a flow rate of 1.2 mL/min, The detection was carried out at 262 nm. The retention time of the Sofosbuvir and Velpatasvir  4.25, 6.05 min respectively. The method produce linear responses in the concentration range of 25-150 µg/mL for Velpatasvir, and 100-600µg/ml of Sofosbuvir. The method precision for the determination of assay was below 2.0%RSD. The proposed method was successfully validated as per ICH validation parameters

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How to Cite
Kumaraswamy, G., Pranay, K., Rajkumar, M., & Lalitha, R. (2017). NOVEL STABILTY INDICATING RP-HPLC METHOD SIMULTANEOUS DETERMINATION OF SOFOSBUVIR AND VELPATASVIR IN BULK AND COMBINED TABLET DOSAGE FORMS. Innovat International Journal Of Medical & Pharmaceutical Sciences, 2(7). https://doi.org/10.24018/10.24018/iijmps.2018.v1i1.22
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Original Article(s)

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