SIMULTANEOUS ESTIMATION OF NEW ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF MEROPENEM AND VABORBACTAM BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY IN BULK AND MARKETED FORMULATION

V. Urukundu; N.M. Vageesh; N.D. Nizamuddin; B Vanaja

doi:10.24018/10.24018/iijmps.2018.v1i1.22

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Published: Jan 1, 2018

DOI: https://doi.org/10.24018/10.24018/iijmps.2018.v1i1.22

Keywords:

Meropenem Vaborbactam RP-HPLC Validation.

V. Urukundu

Department of Pharmaceutical Analysis St.Johns College of pharmaceutical sciences, Yerrakota, Yemmiganur Kurnool (dist) A.P.

N.M. Vageesh

Department of Pharmaceutical Analysis St.Johns College of pharmaceutical sciences, Yerrakota, Yemmiganur Kurnool (dist) A.P.

N.D. Nizamuddin

Department of Pharmaceutical Analysis St.Johns College of pharmaceutical sciences, Yerrakota, Yemmiganur Kurnool (dist) A.P.

B Vanaja

Department of Pharmaceutical Analysis St.Johns College of pharmaceutical sciences, Yerrakota, Yemmiganur Kurnool (dist) A.P.

Abstract

A rapid and precise reverse phase high performance liquid chromatographic method has been developed for the validation of Meropenem and Vaborbactam. by high performance liquid chromatography in bulk and marketed formulation. Chromatography was carried out on a X-terra (4.6 Ã—150mm, 5Âµm particle size)Â 5Âµ column using a mixture of Methanol: Acetonitrile: Water 60:20:20v/v as the mobile phase at a flow rate of 1 ml/min, the detection was carried out at 230nm. The retention time of the Meropenem and Vaborbactam was 2.813, 3.886respectively. The method produce linear responses in the concentration range of 10-50Âµg/ml of Meropenem and 66.6-330Âµg/ml of Vaborbactam. The method precision for the determination of assay was below 1%RSD. The method is useful in the quality control of bulk and pharmaceutical formulations.

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How to Cite

Urukundu, V., Vageesh, N., Nizamuddin, N., & Vanaja, B. (2018). SIMULTANEOUS ESTIMATION OF NEW ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF MEROPENEM AND VABORBACTAM BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY IN BULK AND MARKETED FORMULATION. Innovat International Journal Of Medical & Pharmaceutical Sciences, 3(1). https://doi.org/10.24018/10.24018/iijmps.2018.v1i1.22

Issue

Vol 3 Issue 1 2018

Section

Original Article(s)

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