Main Article Content
A rapid and precise reverse phase high performance liquid chromatographic method has been developed for the validation of Meropenem and Vaborbactam. by high performance liquid chromatography in bulk and marketed formulation. Chromatography was carried out on a X-terra (4.6 Ã—150mm, 5Âµm particle size)Â 5Âµ column using a mixture of Methanol: Acetonitrile: Water 60:20:20v/v as the mobile phase at a flow rate of 1 ml/min, the detection was carried out at 230nm. The retention time of the Meropenem and Vaborbactam was 2.813, 3.886respectively. The method produce linear responses in the concentration range of 10-50Âµg/ml of Meropenem and 66.6-330Âµg/ml of Vaborbactam. The method precision for the determination of assay was below 1%RSD. The method is useful in the quality control of bulk and pharmaceutical formulations.
Dr. Kealey and P.J Haines, Analytical Chemistry, 1stedition, Bios Publisher, (2002), PP 1-7.
A.BraithWait and F.J.Smith, Chromatographic Methods, 5thedition, Kluwer Academic Publisher, (1996), PP 1-2.
Andrea Weston and Phyllisr. Brown, HPLC Principle and Practice, 1st edition,
Academic press, (1997), PP 24-37.
Yuri Kazakevich and Rosario Lobrutto, HPLC for Pharmaceutical Scientists, 1stedition, Wiley Interscience A JohnWiley & Sons, Inc., Publication, (2007), PP 15-23.
Chromatography, (online). URL:http://en.wikipedia.org/wiki/Chromatography.
Meyer V.R. Practical High-Performance Liquid Chromatography, 4th Ed. England, John Wiley & Sons Ltd, (2004), PP 7-8.
Sahajwalla CG a new drug development, vol 141, Marcel Dekker Inc., New York, (2004), PP 421â€“426.
Introduction to Column. (Online),URL:http://amitpatel745.topcities.com/index_files/study/column care.pdf
Detectors used in HPLC (online )URL:http://wiki.answers.com/Q/What_detectors_are_used_in_HPLC
Detectors (online) ,URL:http://hplc.chem.shu.edu/NEW/HPLC_Book/Detectors/det_uvda.html
Detectors (online) ,URL:http://www.dionex.com/enus/webdocs/64842-31644-02_PDA-100.pdf