RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF SOFOSBUVIR AND VELPATASVIR IN PURE AND PHARMACEUTICAL DOSAGE FORM

B Vanaja; N.M. Vageesh; C. Kistayya; V. Urukundu

doi:10.24018/10.24018/iijmps.2018.v1i1.22

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Published: Jan 1, 2018

DOI: https://doi.org/10.24018/10.24018/iijmps.2018.v1i1.22

Keywords:

Sofosbuvir Velpatasvir RP-HPLC validation.

B Vanaja

Dept.of Pharmaceutical Analysis St.Johns College of pharmaceutical sciences, Yerrakota, yemmiganur Kurnool (dist) A.P.

N.M. Vageesh

Dept.of Pharmaceutical Analysis St.Johns College of pharmaceutical sciences, Yerrakota, yemmiganur Kurnool (dist) A.P.

C. Kistayya

Dept.of Pharmaceutical Analysis St.Johns College of pharmaceutical sciences, Yerrakota, yemmiganur Kurnool (dist) A.P.

V. Urukundu

Dept.of Pharmaceutical Analysis St.Johns College of pharmaceutical sciences, Yerrakota, yemmiganur Kurnool (dist) A.P.

Abstract

A rapid and precise Reverse Phase High Performance Liquid Chromatographic method has been developed for the validated of Sofosbuvir and Velpatasvir, in its pure form as well as in tablet dosage form. Chromatography was carried out on a Symmetry C18 (4.6 x 150mm, 5Âµm) column using a mixture of Methanol: TEA pH 4.2 (40:60) as the mobile phase at a flow rate of 1.0ml/min, the detection was carried out at 272 nm. The retention time of the Sofosbuvir and Velpatasvir was 2.781, 4.048 Â±0.02min respectively. The method produce linear responses in the concentration range of 7.5-37.5Âµg/ml of Sofosbuvir and 5-25Âµg/ml of Velpatasvir. The method precision for the determination of assay was below 2.0%RSD. The method is useful in the quality control of bulk and pharmaceutical formulations

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How to Cite

Vanaja, B., Vageesh, N., Kistayya, C., & Urukundu, V. (2018). RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF SOFOSBUVIR AND VELPATASVIR IN PURE AND PHARMACEUTICAL DOSAGE FORM. Innovat International Journal Of Medical & Pharmaceutical Sciences, 3(1). https://doi.org/10.24018/10.24018/iijmps.2018.v1i1.22

Issue

Vol 3 Issue 1 2018

Section

Original Article(s)

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