SIMULTANEOUS ESTIMATION OF NEW ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF GLECAPREVIR AND PIBRENTASVIR BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY.

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K Hemalatha
C Kistayya
ND Nizamuddhin
D Dastiagiriamma

Abstract

A rapid and precise reverse phase high performance liquid chromatographic method has been developed for the validated of Pibrentasvir and Glecaprevir, in its pure form as well as in tablet dosage form. Chromatography was carried out on a Altima C18 (4.6 x 150mm, 5µm) column using a mixture of Methanol: TEA Buffer pH 4.5: Acetonitrile (50:25:25) as the mobile phase at a flow rate of 1.0ml/min, the detection was carried out at 225 nm. The retention time of the Pibrentasvir and Glecaprevir was 2.102, 3.537 ±0.02min respectively. The method produce linear responses in the concentration range of 5-25mg/ml of Pibrentasvir and 12.5-62.5mg/ml of Glecaprevir. The method precision for the determination of assay was below 2.0%RSD. The method is useful in the quality control of bulk and pharmaceutical formulations.

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Hemalatha, K., Kistayya, C., Nizamuddhin, N., & Dastiagiriamma, D. (2018). SIMULTANEOUS ESTIMATION OF NEW ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF GLECAPREVIR AND PIBRENTASVIR BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY. Innovat International Journal Of Medical & Pharmaceutical Sciences, 3(7). https://doi.org/10.24018/10.24018/iijmps.2018.v1i1.22
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Original Article(s)

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