SIMULTANEOUS ESTIMATION OF LESINURAD AND ALLOPURINOL BY USING REVERSE PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY IN API AND MARKETED FORMULATION.

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Published: Feb 1, 2018
DOI: https://doi.org/10.24018/10.24018/iijmps.2018.v1i1.22
Keywords:
Lesinurad Allopurinol RP-HPLC validation.

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D Dastiagiriamma
Department of pharmaceutical analysis, St.Johns college of pharmaceutical sciences, Yerrakota,
C Kistayya
Department of pharmaceutical analysis, St.Johns college of pharmaceutical sciences, Yerrakota,
HM Sowjanya
Department of pharmaceutical analysis, St.Johns college of pharmaceutical sciences, Yerrakota,
K Hemalatha
Department of pharmaceutical analysis, St.Johns college of pharmaceutical sciences, Yerrakota,

Abstract

A rapid and precise reverse phase high performance liquid chromatographic method has been developed for the validated of Lesinurad and Allopurinol, in its pure form as well as in tablet dosage form. Chromatography was carried out on a Zorbax C18 (4.6 x 150mm, 5µm) column using a mixture of Methanol: Phosphate Buffer pH 3.9 (55:45v/v) as the mobile phase at a flow rate of 1.0ml/min, the detection was carried out at 255nm. The retention time of the Lesinurad and Allopurinol was 2.061, 2.462 ±0.02min respectively. The method produce linear responses in the concentration range of 1-5µg/ml of Lesinurad and 100-500µg/ml of Allopurinol. The method precision for the determination of assay was below 2.0%RSD. The method is useful in the quality control of bulk and pharmaceutical formulations.

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How to Cite
Dastiagiriamma, D., Kistayya, C., Sowjanya, H., & Hemalatha, K. (2018). SIMULTANEOUS ESTIMATION OF LESINURAD AND ALLOPURINOL BY USING REVERSE PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY IN API AND MARKETED FORMULATION. Innovat International Journal Of Medical & Pharmaceutical Sciences, 3(7). https://doi.org/10.24018/10.24018/iijmps.2018.v1i1.22
Issue
Vol 3 Suppl 1 2018
Section
Original Article(s)

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