Main Article Content
A rapid and precise reverse phase high performance liquid chromatographic method has been developed for the validated of Lesinurad and Allopurinol, in its pure form as well as in tablet dosage form. Chromatography was carried out on a Zorbax C18 (4.6 x 150mm, 5Âµm) column using a mixture of Methanol: Phosphate Buffer pH 3.9 (55:45v/v) as the mobile phase at a flow rate of 1.0ml/min, the detection was carried out at 255nm. The retention time of the Lesinurad and Allopurinol was 2.061, 2.462 Â±0.02min respectively. The method produce linear responses in the concentration range of 1-5Âµg/ml of Lesinurad and 100-500Âµg/ml of Allopurinol. The method precision for the determination of assay was below 2.0%RSD. The method is useful in the quality control of bulk and pharmaceutical formulations.
Dr. Kealey and P.J Haines, Analytical Chemistry, 1stedition, Bios Publisher, (2002), PP 1-7.
A.BraithWait and F.J.Smith, Chromatographic Methods, 5thedition, Kluwer Academic Publisher, (1996), PP 1-2.
Andrea Weston and Phyllisr. Brown, HPLC Principle and Practice, 1st edition,
Academic press, (1997), PP 24-37.
Yuri Kazakevich and Rosario Lobrutto, HPLC for Pharmaceutical Scientists, 1stedition, Wiley Interscience A JohnWiley & Sons, Inc., Publication, (2007), PP 15-23.
Chromatography, (online). URL:http://en.wikipedia.org/wiki/Chromatography.
Meyer V.R. Practical High-Performance Liquid Chromatography, 4th Ed. England, John Wiley & Sons Ltd, (2004), PP 7-8.
Sahajwalla CG a new drug development, vol 141, Marcel Dekker Inc., New York, (2004), PP 421â€“426.
Introduction to Column. (Online),URL:http://amitpatel745.topcities.com/index_files/study/column care.pdf
Detectors used in HPLC (online )URL:http://wiki.answers.com/Q/What_detectors_are_used_in_HPLC
Detectors (online) ,URL:http://hplc.chem.shu.edu/NEW/HPLC_Book/Detectors/det_uvda.html
Detectors (online) ,URL:http://www.dionex.com/enus/webdocs/64842-31644-02_PDA-100.pdf